| ISBN: | 9781071628027 |
| 语种代码: | eng |
| 个人名称: | Maupin-Furlow, Julie A. |
| 题名: | Deubiquitinases : methods and protocols / edited by Julie A. Maupin-Furlow and Mariola J. Edelmann. |
| 出版发行项: | New York, NY : Humana Press, [2023] |
| 载体形态: | xiii, 300 pages : illustrations (some color) ; 26 cm. |
| 书目附注: | Includes bibliographical references and index. |
| 格式化内容附注: | Characterization of Deubiquitinase Catalytic State Using a Structure-Based Approach -- Synthetic Diubiquitin Fluorogenic Substrates to Study DUBs -- Fluorometric Characterization of DUB Activity: From Single Enzyme Reactions to Live Intact Cell -- Using Activity-Based Proteomics for the Quantification of Deubiquitinases in Animal Tissue -- Design and Synthesis of Ubiquitin-Based Chemical tools with Unnatural Amino Acids for Selective Detection of Deubiquitinases -- High Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity -- Activity-Based Protein Profiling (ABPP) for Cellular Deubiquitinase (DUB) and Inhibitor Profiling at Deep and High-Throughput Levels -- High-Throughput MALDI-TOF Mass-Spectrometry-based Deubiquitylating Enzyme Assay for Drug Discovery -- Bioinformatical Approaches to The Discovery and Classification of Novel Deubiquitinases -- Application of Archaea in Deubiquitinase-Like Enzyme Discovery and Activity Assay -- Characterization of the Ubiquitin and ISG15 Deconjugase Activity of SARS-CoV-1 and 2 Papain-like Protease -- Next-generation Phage Display to Identify Peptide Ligands of Deubiquitinases -- Identification of Deubiquitinase Substrates in Xenopus Egg Extract -- Identification of Deubiquitinase Substrates in Saccharomyces cerevisiae by Systematic Overexpression -- Real-time Deubiquitination Assays using a Free-Ubiquitin Sensor -- A Method to Monitor Activity of SARS-CoV-2 Nsp3 from Cells -- Use of Deubiquitinase Fusion Proteins to Characterize Endocytic Trafficking in Yeast. |